Isifo sentliziyo, esingoyena nobangela wokufa kwezifo ezingasuleliyo, ubukhulu becala sisifo esinxulumene nokwaluphala.Ngokukhula kweminyaka, intliziyo, njengelungu lokumpompa igazi, iya kuguga, kwaye amandla ayo okuphumla kunye nekhontrakthi iya kuncipha, kwaye ngokuthe ngcembe iya kungakwazi ukumpompa igazi kuwo wonke umzimba, oya kuthi ekugqibeleni ukhokelele ekungaphumeleli kwentliziyo. izigulane kwaye zichaphazela kakhulu ubomi obunempilo babantu.
Ukuguga kwentliziyo kubonakala ngokuncipha kwe-contractility ye-cardiac (ukusebenza kwentliziyo), okuya kuhamba kunye nokuncipha kobuninzi beprotheyini kunye nokutshintsha kweprotheyini yokuguqulwa emva kokuguqulwa.
I-SS-31 peptide yi-cardiolipin peroxidase inhibitor kunye ne-mitochondrial ejolise kwi-peptide.Inokuphucula umsebenzi we-ventricle yasekhohlo kunye ne-mitochondria.I-SS-31 peptide inokunciphisa ukungasebenzi kakuhle kwe-mitochondrial kunye nomonakalo we-oxidative kwiiseli ze-trabecular meshwork yabantu.Inokuthintela iiseli ze-iHTM kunye ne-GTM (3) kwixinzelelo lwe-oxidative eqhubekayo eyenziwa yi-H2O2.
I-SS-31 yi-mitochondrial ejolise kwi-anti-aging substance, ebonakaliswe ukuba iyasebenza ekubuyiseleni ukusebenza kwentliziyo yeempuku ezikhulileyo.I-tetrapeptide yokwenziwa edibeneyo kunye ne-membrane yangaphakathi ye-mitochondrial, enokuphucula umsebenzi we-mitochondrial, ukunciphisa ukuveliswa kweentlobo ze-oksijini ezisebenzayo i-ROS, ukunciphisa inqanaba lezinto eziphazamisayo kunye nokuphucula umsebenzi we-diastolic wentliziyo.
Okokuqala, xa kuthelekiswa neempuku ezincinci kunye neempuku ezindala, izazinzulu zafumanisa ukuba ubuninzi beeprotheyini ze-mitochondrial zichatshazelwa ngakumbi kukuguga, kubandakanywa nendlela yokudluliselwa kwesignali ye-mitochondrial, iiprotheyini ezinxulumene ne-oxidative phosphorylation pathway evelisa amandla, kunye neeprotheyini ezinxulumene ne-SIRT ye-signal transduction path ehambelana namandla. i-metabolism kwi-mitochondria.Ukongezelela, iiprotheni ezibalulekileyo ze-troponin kunye ne-tropomyosin, ezidibanisa ngokuthe ngqo ukuchithwa kwe-myocardial, nazo zichatshazelwa ngokucacileyo ngokuguga.Ezi zihambelana ngokusondeleyo nokusebenza kwentliziyo.Okwesibini, xa kuqwalaselwa impembelelo yonyango lwe-SS-31, abaphandi bafumanisa ukuba ubuninzi beprotheyini yeempuku eziphathwayo azibonakali ngathi zihambelana neqela elincinci, kodwa bonke babonisa ukubuyiswa kwendlela yokungasebenzi ngokuguga. njengobuninzi beprotheyini yomjikelo we-tricarboxylic acid, eyona ndlela iphambili yemveliso yamandla emzimbeni, ethe yachacha ngokwenene ukuya kuthi ga kwinqanaba elikhulu, isenza iimpuku ezindala zibe zincinci.Oku kuthetha ukuba i-SS-31 iyasebenza ngakumbi kutshintsho lwemetabolism yamandla ebangelwa kukuguga kwentliziyo.Ukuphononongwa kobuninzi beprotheyini kwafikelela esiphelweni, emva koko abaphandi bajika ingqalelo yabo kutshintsho kwiprotein emva kokuguqulwa koguqulelo ngexesha lenkqubo yokuguga, kwaye bakhetha ngokukodwa ukuguqulwa okuqhelekileyo emva kokuguqulelwa kwiprotheni, ehambelana kakhulu nentliziyo. -ukuguqulwa kwe-acetylation.Kunokubakho iinguqu ezimbini ekuguqulweni kwe-acetylation.Okokuqala, ngenxa yokuba i-acetylation yeprotheni ye-mitochondrial iya kwanda ngokuguga, okubangela ukungasebenzi kwe-mitochondrial, kunye nomxholo we-mitochondrial wentliziyo uphezulu kakhulu, ngoko yonke intliziyo ingaba ne-acetylation ephezulu yokuqokelela ngelixa umsebenzi wenhliziyo uyancipha;Okwesibini, kuya kubakho ukulahleka kwe-acetylation eqhelekileyo yeentsalela ezithile kwinkqubo yokuguga, eya kubangela ukungaphumeleli kokudlala umsebenzi wayo oqhelekileyo.Abaphandi baye batyebisa i-acetylated peptides entliziyweni (enokuthi iqondwe ngokulula njengeeyunithi ezincinci ezisetyenziselwa ukwenza iprotheni).Kusekho ukungafani kwimo ye-acetylation yeeprotheni zentliziyo phakathi kweqela elincinane kunye neqela elidala, kodwa akubonakali njengobuninzi beprotheni.Emva koko baphinde bahlolisise ukuba zeziphi iiprotheyini ezi tshintsho kwimeko ye-acetylation enokuthi ibe yinto ethile.Ekugqibeleni, abaphandi baphinda badibanisa amandla e-systolic kunye ne-diastolic yentliziyo kwaye bafumana iindawo ze-acetylation ze-14 ezinxulumene nekhono le-diastolic yentliziyo, kwaye zonke zihambelana kakubi.Ngexesha elifanayo, iindawo ezimbini ezinxulumene ne-contractility yenhliziyo nazo zafunyanwa.Oku kuthetha ukuba utshintsho lwe-contractility ngexesha lokuguga lunokulawulwa yi-acetylation state of heart protein.
Singumenzi wepolypeptide eTshayina, esineminyaka eliqela yamava aqolileyo kwimveliso yepolypeptide.I-Hangzhou Taijia Biotech Co., Ltd. yingcali yomenzi we-polypeptide ekrwada, enokubonelela amashumi amawaka ezinto ezikrwada ze-polypeptide kwaye inokwenziwa ngokweemfuno.Umgangatho weemveliso ze-polypeptide ugqwesileyo, kwaye ukucoceka kunokufikelela kwi-98%, eye yaqatshelwa ngabasebenzisi emhlabeni wonke.
